RF-O2熒光光纖氧氣測量技術(shù)是基于REDFLASH光極傳感器技術(shù)的的氧氣測量技術(shù)，由歐洲Pyroscience公司及Graz大學(xué)等科學(xué)家研制生產(chǎn)，由光極氧氣傳感器、測量儀及軟件組成，廣泛應(yīng)用于環(huán)境科學(xué)、生態(tài)科學(xué)、植物科學(xué)、動(dòng)物科學(xué)、海洋科學(xué)、生物醫(yī)學(xué)、生物技術(shù)、食品科學(xué)等各個(gè)領(lǐng)域，其主要功能特點(diǎn)如下

1)REDFLASH光極氧氣傳感器技術(shù)，高精確度、高穩(wěn)定性、高時(shí)空解析度、低能耗、無耗氧、無交叉敏感性

2)傳感器類型靈活多樣，有探頭式、探針式、非接觸式（sensor spot）及納米微粒式等，適應(yīng)于液體和氣體不同條件下的O2測量
3)有內(nèi)置sensor spot的流通管和呼吸瓶，非接觸式測量流動(dòng)液體的溶解氧及呼吸瓶內(nèi)液體或氣體中氧氣含量
4)輕便緊湊型FireStingO2測量儀，內(nèi)置水汽、氣壓傳感器，有1、2、4通道供選配，可分別接1個(gè)、2個(gè)、4個(gè)光極氧氣傳感器，另有Mini型FireStingO2-mini供選配
5)U盤式PiccolO2測量儀——世界上小的O2測量儀，可連接一個(gè)O2傳感器，USB口連接電腦，即插即用
測量原理：

REDFLASH光極O2傳感器技術(shù)，利用獨(dú)特的O2敏感REDFLASH指示劑，通過610-630nm調(diào)制紅光激發(fā)，REDFLASH指示劑發(fā)出760-790nm紅外熒光，熒光強(qiáng)度隨接觸的O2分子濃度升高而發(fā)生熒光淬滅，這種熒光動(dòng)態(tài)通過光纖傳輸?shù)綔y量儀，測量儀靈敏地檢測其相位漂移并據(jù)此換算成O2濃度

應(yīng)用領(lǐng)域：
1)水體溶解氧測量監(jiān)測、藻類及藻類生物膜光合作用與呼吸作用測量監(jiān)測
2)植物光合作用與呼吸作用測量監(jiān)測
3)水生動(dòng)物（魚類、水生昆蟲等無脊椎動(dòng)物、浮游動(dòng)物等呼吸代謝測量
4)陸生動(dòng)物、實(shí)驗(yàn)動(dòng)物、動(dòng)物組織、血液等呼吸代謝測量
5)土壤、濕地、海洋沉積、河湖沉積剖面O2測量
6)生物反應(yīng)器、發(fā)酵過程、酶動(dòng)力學(xué)、細(xì)胞培養(yǎng)等O2測量監(jiān)測
7)糧食食品儲(chǔ)運(yùn)、葡萄酒等O2測量監(jiān)測
8)污水處理、沼氣、垃圾填埋場、有機(jī)物降解等O2測量監(jiān)測
技術(shù)指標(biāo)：
1)FireStingO2（FSO2）測量儀：
a)有1通道、2通道、4通道可供選配，分別可接1個(gè)、2個(gè)和4個(gè)O2傳感器，可并聯(lián)組成8通道甚至更多通道；另具備一個(gè)溫度傳感器通道（可選配4通道溫度傳感器）
b)激發(fā)光源620nm，監(jiān)測器760nm（NIR）
c)采樣頻率：每秒4次
d)內(nèi)置氣壓傳感器，300－1100mbar，0.06mbar分辨率，精確度±3mbar
e)內(nèi)置濕度傳感器，0－，分辨率0.04%，精確度±0.2%
f)內(nèi)置溫度傳感器，-40－125°C，分辨率0.01°C，精確度±0.3°C
g)具模擬輸出和自動(dòng)模式，0－2.5VDC
h)USB接口，通過USB口PC供電
i)大?。?8x120x30mm，重350g

2)PiccolO2 U盤式測量儀：大小僅15x15x54mm，重量約20g，單通道，激發(fā)光620nm，檢測器760nm，采樣頻率每秒20次。可并聯(lián)組成多通道測量系統(tǒng)?？赏ㄟ^PiccoTHP測量溫濕度和氣壓并進(jìn)行補(bǔ)償  

3)探頭式O2傳感器：直徑3mm，測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，使用壽命1千萬數(shù)據(jù)點(diǎn)，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）

4)探針式O2傳感器：有固定探針式、可伸縮探針式、尖頭式及圓頭式等不同類型供選配；探針直徑有50μm、230μm、430μm等規(guī)格；測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，快響應(yīng)時(shí)間小于1s（與探針粗細(xì)有關(guān)），使用壽命1百萬數(shù)據(jù)點(diǎn)，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）

  

5)非接觸式（sensor spot）O2傳感器（見下左圖）：用于非接觸性測量監(jiān)測透明容器中的氧氣含量，傳感器貼用硅膠等貼附在容器內(nèi)壁，通過固定在外壁的光纖將熒光動(dòng)態(tài)信號(hào)傳輸?shù)綔y量儀以檢測O2濃度；測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，使用壽命2千萬數(shù)據(jù)點(diǎn)，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）  

6)納米微粒傳感器（參見上右圖）：納米技術(shù)，用于非接觸性測量微量液體中O2含量，即時(shí)響應(yīng)，測量范圍0-50%（0-23mg/l），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）
7)流通管：內(nèi)置非接觸式O2傳感器，用于流動(dòng)液體O2測量監(jiān)測（如魚類呼吸代謝測量等），測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，使用壽命1千萬數(shù)據(jù)點(diǎn)，存儲(chǔ)時(shí)間大于3年（室溫暗處儲(chǔ)放）
8)呼吸瓶：內(nèi)置非接觸式O2傳感器，用于生物呼吸測量（如藻類、小型魚類、魚卵、昆蟲等），標(biāo)準(zhǔn)配置有4ml和20ml兩種規(guī)格

9)Pyro Oxygen Logger軟件用于參數(shù)設(shè)置、校準(zhǔn)、數(shù)據(jù)顯示包括圖表顯示、數(shù)據(jù)輸出等功能

應(yīng)用案例：
案例1：法國Bordeaux大學(xué)利用FSO2 4通道熒光光纖氧氣測量儀，對Aquitaine海岸沉積樣芯耗氧進(jìn)行了測量分析，以研究海洋底棲動(dòng)物活動(dòng)（bioirrigation）對海岸帶生態(tài)系統(tǒng)生態(tài)過程及生物地理化學(xué)功能（如沉積有機(jī)物的再礦化）的影響。
案例2：芬蘭Turku大學(xué)利用FSO2和430μm光極氧探針，對南瓜類囊體懸浮液光合放氧進(jìn)行了測量分析。
案例3：美國Woods Hole海洋學(xué)研究所，利用RF-O2非接觸式光極氧氣傳感器（sensor spot），對海洋無脊椎動(dòng)物呼吸代謝進(jìn)行了測量分析，以研究其固有的生物鐘與環(huán)境脅迫的關(guān)系，這些海洋無脊椎動(dòng)物體重只有0.5－50mg。圖中為翼足類軟體動(dòng)物在不同濃度CO2條件下的耗氧率。

案例4：澳大利亞海洋科學(xué)研究所、瑞典Gothenburg大學(xué)等組成的科學(xué)小組，利用Pyroscience的REDFLASH氧氣測量技術(shù)，對河鱸（Perca fluviatilis）呼吸代謝進(jìn)行測量分析，以研究其熱耐受性和適應(yīng)性的生理機(jī)制。他們選擇波羅的海核電站附近的一個(gè)瀉湖，核電站排出的熱水進(jìn)入該瀉湖，在過去30年大量魚類因?yàn)椴贿m應(yīng)水溫升高而滅絕，但河鱸卻得以繁盛，該地成為理想的研究氣候變暖對魚類種群影響的“天然實(shí)驗(yàn)室”。他們測量河鱸呼吸代謝率的同時(shí)，還測量其靜脈血液在溫度升高狀態(tài)下的氧分壓，靜脈血是河鱸心臟供氧的主要來源，高溫條件下靜脈血氧氣含量被認(rèn)為是其心臟功能的重要限制因子。

案例5：德國Ulm大學(xué)利用FSO2測量儀和50μm可伸縮式RFO2探針，對患者腦脊髓液（CSF）樣品溶解氧進(jìn)行測量分析，以研究探討神經(jīng)紊亂及神經(jīng)炎等疾病的生理和診斷。
案例6：德國農(nóng)業(yè)科學(xué)與景觀研究機(jī)構(gòu)，利用FSO2測量儀和RFO2探針，對土壤氧氣進(jìn)行測量，以評估不同種類蚯蚓在低氧條件下對土壤改良的效率。

案例7：西班牙Valladolid大學(xué)利用RFO2熒光光纖氧氣測量技術(shù)，監(jiān)測葡萄酒橡木桶O2吸收——對葡萄酒品質(zhì)至關(guān)重要但一直以來缺乏科學(xué)的了解。葡萄酒在橡木桶內(nèi)（3－24個(gè)月）的過程溶解氧至關(guān)重要，因?yàn)镺2調(diào)節(jié)了葡萄酒整個(gè)的熟化過程。

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